Objective To provide a preliminary evaluation of the self?made horseradish peroxidase (HRP) marked Hantavirus (HV) recombinant N protein (rNP) rNP?IgM direct capture ELISA diagnostic kit. Methods Assessment of the specificity and stability of the kit and comparison of clinical results with similar products were performed to evaluate the sensitivity of the kit in the detection of serum anti?HV?IgM. Results (1) The kit was only responsive to anti?HV?IgM positive serum, and irresponsive to anti?varicella?zoster virus?IgM (anti?VZV?IgM), anti?Japanese encephalitis virus?IgM (anti?JEV?IgM), anti?dengue virus?IgM (anti?DV?IgM), anti?hand, foot and mouth EV71 virus?IgM (anti?EV71?IgM) positive sera. No obvious reduction in serum anti?HV?IgM detecting capability was noticed after placement at 37 ℃ for 3 d. (2) In 144 sera samples in 120 patients with suspected hemorrhagic fever with renal syndrome, inconsistency was observed only in the anti?HV?IgM test results in 12 sera of 12 patients between the two kinds of kits, in which 8 primary sera samples were detected positive by the kit and negative by commercial ones (the secondary sera samples were positive for both kits); 3 primary samples (the secondary samples unavailable) were at the critical value for the kit and negative for commercial kits. Meanwhile, one other primary serum sample was positive for the kit and negative for the commercial ones (the secondary and tertiary ones positive for both). Conclusion The laboratory developed capture ELISA anti?HV?IgM diagnostic kits had favorable specificity, stability and sensitivity, suitable for the clinical diagnosis and epidemiological surveillance of HV infections.

【Abstract】 Objective Explore the role of chigger mite in the transmission of hemorrhagic fever with renal syndrome (HFRS). Methods Chigger mites were collected from rats caught in the plague foci. HV was detected by Vero-E6 culture and IFA. HV-RNA from mites was tested by RT-PCR, and HV was located by hybridization histochemistry.  Results The infection rate of HV was 66.7% in chigger mites collected from rats which antigens were positive, however it was only 25.0% in mites from rats which antigens were negative. The result showed that chigger mite could transmit HFRS by stinging and vertical transmission. Conclusion It indicates that chigger mite is one of HFRS vectors, which roles in HFRS transmission can not be neglected. 

【Abstract】 Objective To clarify the natural infection situation of rodents by Hantavirus (HV) and HV strains in Zhejiang province in 2007，and provide science evidence for the prevention and control of hemorrhagic fever with renal syndrome (HFRS)．Methods Lung tissue and serum from rodent were sampled, and IgG antibody from serum was tested by indirect immunofluorscence assay and direct immunofluorscence assay was adopted to test HV antigens from lung tissues. HV was isolated by Vero?E6 cells, and HV strains were identified by Anti?McAb．Results A total of 1129 rodents were captured in 2007．The dominant specie was Apodemus agrarius, and the positive rate of HV antigen in rodent lungs was 3.0%. The IgG antibody of 57 blood samples was positive, and the positive rate was 8.0%.  Six  strains  of  hantaan (HTN)  virus  were  isolated,  five  strains  from A.agrarius  and  one  from  Rattus norvegicus.  Conclusion There  are  natural  foci  of  HFRS  which  main  infection  sources  are A.agrarius in Zhejiang province, and HTN strain could be the main prevalence strains of HV.